Electrostatic Effects in Filamentous Protein Aggregation

Electrostatic Modeling of Protein Aggregation

Ram Vanam M.S., Indiana University, December 2004. Electrostatic modeling of protein aggregation. Research Advisor: Paul L. Dubin Electrostatic modeling was done with Delphi of insight II to explain and predict protein aggregation, measured here for β-lactoglobulin and insulin using turbidimetry and stopped flow spectrophotometry. The initial rate of aggregation of β-Lactoglobulin was studied b...

Electrostatic Effects in Aggregation of Crystallin Proteins

Beta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells

Background: The canonical Wnt signal transduction (or the Wnt/β-catenin pathway) plays a crucial role in the development of animals and in carcinogenesis. Beta-catenin is the central component of this signaling pathway. The activation of Wnt/β-catenin signaling results in the cytoplasmic and nuclear accumulation of β-catenin. In the nucleus, β-catenin interacts with the TCF/LEF transcription fa...

Electrostatic Control of Spontaneous Vesicle Aggregation

Aqueous mixtures of cationic cetyltrimethylammonium p-toluenesulfonate (CTAT) and anionic sodium dodecylbenzenesulfonate (SDBS)spontaneously formequilibrium“catanionic”vesicleswhosechargedepends on the relative amounts of each surfactant. Aggregation of these vesicles was induced by incorporating a small concentration of biotin-lipid in the bilayer, followed by addition of streptavidin as a cro...

Modeling electrostatic effects in proteins.

Electrostatic energies provide what is perhaps the most effective tool for structure-function correlation of biological molecules. This review considers the current state of simulations of electrostatic energies in macromolecules as well as the early developments of this field. We focus on the relationship between microscopic and macroscopic models, considering the convergence problems of the m...